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Journal: Cell reports
Article Title: The adiponectin-PPARγ axis in hepatic stellate cells regulates liver fibrosis
doi: 10.1016/j.celrep.2024.115165
Figure Lengend Snippet: (A–K) Control (Lrat-rtTA) and LAPNTG (Lrat-rtTA+TRE-Adipoq) mice were housed under thermoneutral conditions and fed HFD+DOX to induce liver fibrosis. (A) Experimental setup. (B) Adiponectin mRNA expression in HSCs after 2 weeks’ doxycycline. (C) Circulating adiponectin levels after 2 weeks’ doxycycline ( n = 5). (D) Body weight development. (E) OGTT after 8 weeks ( n = 5–8). (F–J) Liver mRNA expression of (F) Col1a1, (G) Col3a1, (H) Col4a4, (I) TGF-β, and (J) smooth muscle actin after 1 year under thermoneutral conditions. (K) Liver trichrome staining. Scale bar represents 100 μm. (L) Picrosirius red staining. Scale bar represents 100 μm. (M) Quantification of fibrotic area. (N–U) Control (MUP-uPA+Lrat-rTA) and MUPLAPNTG (MUP-uPA+Lrat-rtTA+TRE-Adipoq) mice were fed chow+DOX. (N) Experimental setup. (O–Q) Liver mRNA expression of (O) smooth muscle actin, (P) Col3a1, and (Q) Col4a4 after 20 weeks. (R) Liver trichrome staining after 20 weeks ( n = 10). (S) Picrosirius red staining. (T) Quantification of fibrotic area. (U) Liver tumors after 20 weeks’ HFD plus Dox600 ( n = 10). Data are displayed as mean ± SEM and analyzed by unpaired Student’s t test for all the figures: * p < 0.05, ** p < 0.01, *** p < 0.001.
Article Snippet:
Techniques: Control, Expressing, Staining
Journal: Cell reports
Article Title: The adiponectin-PPARγ axis in hepatic stellate cells regulates liver fibrosis
doi: 10.1016/j.celrep.2024.115165
Figure Lengend Snippet:
Article Snippet:
Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, cDNA Synthesis, SYBR Green Assay, Generated, Software, Fluorescence, Microscopy